A recombinant DNA molecule was created by ligating a gene to a plasmid vector.

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asked Mar 13, 2018 in Class XII Biology by nikita74 (-1,032 points)
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation ?

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answered Mar 13, 2018 by vijay Premium (530 points)
The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.

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