Free Study Rankers App Download Now
0 votes
in Class XII Biology by (-1,017 points)
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation ?

Please log in or register to answer this question.

1 Answer

0 votes
by Premium (539 points)
The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.

Related questions