Would you choose an exonuclease while producing a recombinant DNA molecule ?

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asked Mar 13, 2018 in Class XII Biology by nikita74 (-1,028 points)
Would you choose an exonuclease while producing a recombinant DNA molecule?

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answered Mar 13, 2018 by rahul152 (-2,846 points)
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.

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