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Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts ?
(a) Laboratory flask of largest capacity
(b) A stirred-tank bioreactor without inlets and outlets
(c) A continuous culture system
(d) Any of the above

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• After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein one has to consider producing it on a large scale. Small volume cultures cannot yield appreciable quantities of products.
• To produce in large quantities the development of bioreactors, where large volume (100-1000 litres) of culture (continuous) can be processed, was required.

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